Searchable abstracts of presentations at key conferences on calcified tissues

ba0007p219 | (1) | ICCBH2019

Comparison of cell separation methods, using relative expression of specific growth plate zone markers in a pig model

Javanmardi Alireza , Raimann Adalbert , Egerbacher Monika , Sagmeister Susanne , Gleiss Andreas , Haeusler Gabriele

Objective: Linear growth is achieved by enchondral ossification in epiphyseal growth plates (GP) of long bones. These highly organized cartilaginous tissues contain chondrocytes of all differentiational stages classified in 3–5 specific zones. Due to their discrete characteristics, distinct analysis of each zone is essential in basic GP research. While the efficiency of zonal separation is therefore highly influencing on study results, comparative data on commonly used me...